General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS115RTA)
The Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line, 2 vials. (HTS117C)
The ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen M3 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS116M)
The ChemiScreen M3 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM3 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen ETA Endothelin Receptor Stable Cell Line, 2 vials. (HTS070C)
The ChemiScreen ETA Endothelin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin) based activation of EDNRA GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDNRA alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRA, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen FFA2/GPR43 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS063C)
The ChemiScreen FFA2/GPR43 Free Fatty Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CXCR6 Chemokine Receptor Membrane Preparation, 1 vial. (HTS054M)
The ChemiScreen CXCR6 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CXCR6 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen CXCR1 Chemokine Receptor Membrane Preparation, 1 vial. (HTS001M)
The ChemiScreen CXCR1 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CXCR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC HCT116-SLC25A14-KO-c11; Colorectal carcinoma; Human(Homo sapiens)
The HCT116-SLC25A14-KO-c11 cell line contains a 1 bp insertion in SLC25A14 gene and combined with the parental cell line and other HCT116 derivatives can be used in cancer drug screening.
ATCC HCT116-SLC10A4-KO-c2; Colorectal carcinoma; Human (Homo sapiens)
The HCT116-SLC10A4-KO-c2 cell line contains a +1 bp insertion in SLC10A4 gene and combined with the parental cell line and other HCT116 derivatives can be used in cancer drug screening.
ATCC Staphylococcus aureus; subsp. aureus
Staphylococcus aureus subsp. aureus strain Wood 46 is a whole-genome sequenced bacterium with applications in dermatological research. This strain is known to produce dermatoxin.
ATCC Fusarium verticilliodes; H66173
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
ATCC MDCK.STAT1KO; CRISPR/Cas9; Mod. Cell Line; Dog kidney
MDCK.STAT1 KO cellline was CRISPR/Cas9 genome edited to significantly increase your viral vaccine production capacity.
